Journal: ACS Central Science
Article Title: Protease Activated Probes for Real-Time Ratiometric Imaging of Solid Tumors
doi: 10.1021/acscentsci.3c00261
Figure Lengend Snippet: Development and preclinical testing of the ratiometric camera system. (a) Photo and schematic of the components of the camera. (b) Representative images acquired with the camera system showing RATIO channel of vials containing 6QC-RATIO [100 nM] which has been incubated for 30 min with either vehicle, cathepsin L [20 nM], or cathepsin L [20 nM] preincubated 30 min with cathepsin inhibitor E64d [10 μM]. (c) schematic of experimental setup for ratiometric imaging. (d) Representative RATIO camera images showing a mouse carrying two breast tumors injected with Death-Cat-RATIO [10 nmol] 24 h prior to imaging. From left to right, the panels show the brightfield image, the overlay between the Cy5 channel and the brightfield image, and the overlay between the RATIO channel and the brightfield image. The tumor closer to the camera (area 1) is located in the third mammary fat pad; the tumor further from the camera (area 2) is located in the eighth mammary fat pad. Scale bars: 1 cm. (e) Representative RATIO camera images of a mouse model of small 4T1 lesions. Representative white light photo of a splayed mouse (left) and overlay of the RGB signal and RATIO signal acquired with the ratiometric camera (right). (f) Photo of the da Vinci setup for ratiometric robotic surgery with view of the subject stage. (g) Representative images of ratiometric robotic surgery carried out in mice injected with Death-Cat-RATIO [10 nmol] 24 h prior to imaging. Images were acquired with the custom camera system and show, from left to right, the RGB channel, the Cy5 channel, the RATIO channel, and an overlay between the RGB and RATIO channel. Scale bars: 1 cm.
Article Snippet: To evaluate the performance of ratiometric optical contrast agents in vivo , we employed the 4T1 orthotopic breast cancer mouse model. We injected 4T1 (ATCC CRL-2539) mammary carcinoma cells subcutaneously into the third and eighth mammary fat pads.
Techniques: Incubation, Imaging, Injection
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![Development and preclinical testing of the ratiometric camera system. (a) Photo and schematic of the components of the camera. (b) Representative images acquired with the camera system showing RATIO channel of vials containing 6QC-RATIO [100 nM] which has been incubated for 30 min with either vehicle, cathepsin L [20 nM], or cathepsin L [20 nM] preincubated 30 min with cathepsin inhibitor E64d [10 μM]. (c) schematic of experimental setup for ratiometric imaging. (d) Representative RATIO camera images showing a mouse carrying two breast tumors injected with Death-Cat-RATIO [10 nmol] 24 h prior to imaging. From left to right, the panels show the brightfield image, the overlay between the Cy5 channel and the brightfield image, and the overlay between the RATIO channel and the brightfield image. The tumor closer to the camera (area 1) is located in the third mammary fat pad; the tumor further from the camera (area 2) is located in the eighth mammary fat pad. Scale bars: 1 cm. (e) Representative RATIO camera images of a mouse model of small <t>4T1</t> lesions. Representative white light photo of a splayed mouse (left) and overlay of the RGB signal and RATIO signal acquired with the ratiometric camera (right). (f) Photo of the da Vinci setup for ratiometric robotic surgery with view of the subject stage. (g) Representative images of ratiometric robotic surgery carried out in mice injected with Death-Cat-RATIO [10 nmol] 24 h prior to imaging. Images were acquired with the custom camera system and show, from left to right, the RGB channel, the Cy5 channel, the RATIO channel, and an overlay between the RGB and RATIO channel. Scale bars: 1 cm.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_4504/pmc10214504/pmc10214504__oc3c00261_0006.jpg)